ELISA检测限(ng/well)提高8-156倍
检测限测定方法：包被一系列倍比稀释的抗原（重组蛋白），加上1ug/mL（mouse mAb）或者0.1ug/mL（rabbit mAb）抗体，再用相应的酶标二抗进行检测，每个浓度5个重复。
结果的判定：检测浓度的平均OD值减去3SD>空白的平均OD值加上3SD，最低的检出浓度即为检测限。
Western Blot检测限达到ng或pg级
实验中，抗体稀释度更高
| Antibody Types | Application | |
| ELISA | Western Blot | |
| Rabbit mAb | 0.1-0.2 | 1-2 |
| Mouse mAb | 0.5-1 | 1-2 |
| Rabbit pAb (affinity purified) | 0.5-1 | 0.1-0.2 |
Affordable Price
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Dramatic cost saving for quality antibodies.
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Big discount for bulk order.
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Please contact us for a bulk order quote.