流感病毒核蛋白（NP）在病毒复制和宿主适应中起关键作用，属于结构蛋白。针对NP蛋白的抗体通常用于各种流感病毒的免疫检测中，如ELISA、FLA、荧光抗体检测等。这些检测可用于基础研究和临床诊断。由于不同流感毒株之间抗原漂移或转移的频率很高，因此在流感诊断中特别需要广谱流感抗体。

义翘神州已鉴定出6对广谱抗体，分别针对甲型流感和乙型流感的NP蛋白。通过夹心法ELISA检测，这些抗原-抗体配对具有高灵敏度，可达到皮克级（pg）。

Antibody Pairs for Pan Influenza A NP Detection Antibody Pairs Capture Ab Detection Ab PanA-1 40205-MM16 40208-R014 PanA-2 40205-R063 40205-MM18 PanA-3 40208-R117 40205-MM18

Antibody Pairs for Pan Influenza B NP Detection Antibody Pairs Capture Ab Detection Ab PanB-1 40438-R004 40438-MM10 PanB-2 40438-R036 40438-MM10 PanB-3 40438-MM05 40438-R036

High Sensitivity (pg level)

Broad Spectrum and High Specificity

These antibody pairs can detect a broad spectrum of strains within the target subtype, without any cross-reactivity with the other subtypes.
24 kinds of influenza antigens from A or B have been tested by ELISA assay

The Aforementioned Antigens:

B-1:	40783-V08B (B, B/Brisbane/60/2008)	A-8:	40774-V08B (H1N1, A/Victoria/2570/2019 | A/Wisconsin/588/2019)
B-2:	40782-V08B (B, B/Colorado/06/2017)	A-9:	40033-V08B (H2N2, A/Ann Arbor/6/1960)
B-3:	40438-V08B (B, B/Florida/4/2006)	A-10:	40207-V08B (H3N2, A/Aichi/2/1968)
B-4:	40500-V08B (B, B/Phuket/3073/2013)	A-11:	40778-V08B (H3N2, A/Cambodia/e0826360/2020 (H3N2)-like)
B-5:	40755-V08B (B, B/Washington/02/2019)	A-12:	40208-V08B (H3N2, A/Hong Kong/1/1968)
A-1:	40204-V08B (H1N1, A/Brevig Mission/1/1918)	A-13:	40753-V08B (H3N2, A/Hong Kong/2671/2019)
A-2:	40776-V08B (H1N1, A/Brisbane/02/2018)	A-14:	40754-V08B (H3N2, A/Hong Kong/45/2019)
A-3:	40205-V08B (H1N1, A/California/07/2009)	A-15:	40781-V08B (H3N2, A/Hong Kong/4801/2014 )
A-4:	40723-V08B (H1N1, A/Guangdong-Maonan/SWL1536/2019)	A-16:	40779-V08B (H3N2, A/Kansas/14/2017)
A-5:	40724-V08B (H1N1, A/Hawaii/70/2019)	A-17:	40499-V08B (H3N2, A/Switzerland/9715293/2013)
A-6:	40777-V08B (H1N1, A/Michigan/45/2015)	A-18:	40110-V08B (H7N9, A/Anhui/1-BALF_RG6/2013)
A-7:	11675-V08B (H1N1, A/Puerto Rico/8/34/Mount Sinai)	A-19:	40111-V08B (H7N9, A/Shanghai/2/2013)

High Binding Affinity

The binding affinity between immobilized Influenza B NP protein (Cat#: 40500-V08B) with the anti-NP antibody (Cat#: 40438-R036) in BLI assay.

The binding affinity between immobilized Influenza A NP protein (Cat#: 40774-V08B) with the anti-NP antibody (Cat#: 40205-R063) in BLI assay.

Antibody Cocktail Can Successfully Detect both A and B Type Flu The combination of A and B antibody pairs can allow detection of both subtypes.

High Production Quality Our products and processes are ISO13485 certified. The purity of these antibodies is >95% as assessed by SEC-HPLC.

Other Pan Influenza NP Antibodies

16 Pan Influenza A or B Nucleoprotein antibodies have been developed and validated by ELISA and WB assays.

Pan Influenza A NP Antibodies

40208-MM03		40208-R014		40208-MM04		40208-R010		40208-R061
40208-R113		40208-R117		40205-MM16		40205-MM18		40205-R063

Pan Influenza B NP Antibodies

40438-MM05

40438-R016

40438-MM09

40438-R036

40438-R004

40438-MM10

Western Blot Analysis of Nucleoprotein Antibodies to 24 Influenza Antigens

Lane 1: 40204-V08B | H1N1 (A/Brevig Mission/1/1918)
Lane 2: 40776-V08B | H1N1 (A/Brisbane/02/2018)*
Lane 3: 40205-V08B | H1N1 (A/California/07/2009)
Lane 4: 40723-V08B | H1N1 (A/Guangdong-Maonan/SWL1536/2019)*
Lane 5: 40724-V08B | H1N1 (A/Hawaii/70/2019)*
Lane 6: 40777-V08B | H1N1 (A/Michigan/45/2015)*
Lane 7: 11675-V08B | H1N1 (A/Puerto Rico/8/34/Mount Sinai)
Lane 8: 40774-V08B | H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019)
Lane 9: 40033-V08B | H2N2 (A/Ann Arbor/6/1960)
Lane 10: 40207-V08B | H3N2 (A/Aichi/2/1968)
Lane 11: 40778-V08B | H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like)
Lane 12: 40208-V08B | H3N2 (A/Hong Kong/1/1968)
Lane 13: 40753-V08B | H3N2 (A/Hong Kong/2671/2019)*
Lane 14: 40754-V08B | H3N2 (A/Hong Kong/45/2019)*
Lane 15: 40781-V08B | H3N2 (A/Hong Kong/4801/2014)*
Lane 16: 40779-V08B | H3N2 (A/Kansas/14/2017)*
Lane 17: 40499-V08B | H3N2 (A/Switzerland/9715293/2013)*
Lane 18: 40110-V08B | H7N9 (A/Anhui/1-BALF_RG6/2013)
Lane 19: 40111-V08B | H7N9 (A/Shanghai/2/2013)
Lane 20: 40783-V08B | Influenza B (B/Brisbane/60/2008)*
Lane 21: 40782-V08B | Influenza B (B/Colorado/06/2017)*
Lane 22: 40438-V08B | Influenza B (B/Florida/4/2006)
Lane 23: 40500-V08B | Influenza B (B/Phuket/3073/2013)*
Lane 24: 40755-V08B | Influenza B (B/Washington/02/2019)*

*: Antigen from 2015-2022 Influenza Vaccine Strain

Pan Influenza A and B Hemagglutinin Antibody

Because of the distinct role of HA, it is widely used for research on antibodies against influenza. WB and ELISA assay have been performed to confirm the recognition of HA antigens. Results show that rabbit Mab 86001-RM01 and 11053-R004 are broad spectrum antibodies for Influenza A and B, respectively.

Western Blot Analysis of Hemagglutinin Antibodies to 20 Influenza Antigens

Anti-Influenza A HA rabbit mAb (Cat#: 86001-RM01) at 1:5000 dilution.

Anti-Influenza B HA rabbit mAb (Cat#: 11053-R004) at 1:10000 dilution.

Lane 1: 40719-V08H | H1N1 (A/Brisbane/02/2018) *
Lane 2: 11055-V08H | H1N1 (A/California/04/2009)
Lane 3: 11085-V08B | H1N1 (A/California/07/2009)*
Lane 4: 40717-V08H | H1N1 (A/Guangdong-Maonan/SWL1536/2019)*
Lane 5: 40567-V08H1 | H1N1 (A/Michigan/45/2015)*
Lane 6: 11684-V08H | H1N1 (A/Puerto Rico/8/1934)
Lane 7: 40787-V08H | H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) *
Lane 8: 11056-V08H | H3N2 (A/Brisbane/10/2007)
Lane 9: 40789-V08H | H3N2 (A/Cambodia/e0826360/2020)*
Lane 10: 40721-V08H | H3N2 (A/Hong Kong/2671/2019)*
Lane 11: 40765-V08H | H3N2 (A/Hong Kong/45/2019)*
Lane 12: 40555-V08B | H3N2 (A/Hong Kong/4801/2014)*
Lane 13: 40720-V08H | H3N2 (A/Kansas/14/2017) *
Lane 14: 40580-V08H | H3N2 (A/Singapore/INFIMH-16-0019/2016)*
Lane 15: 40497-V08B | H3N2 (A/Switzerland/9715293/2013)*
Lane 16: 40016-V08B | Influenza B (B/Brisbane/60/2008)*
Lane 17: 40581-V08H | Influenza B (B/Colorado/06/2017)*
Lane 18: 11053-V08H | Influenza B (B/Florida/4/2006)
Lane 19: 40498-V08B | Influenza B (B/PHUKET/3073/2013)*
Lane 20: 40722-V08H Influenza B (B/Washington/02/2019)*

*: Antigen from 2015-2022Influenza Vaccine Strain