SEC-MALS验证的新冠突变株三聚体蛋白_公司新闻

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北京义翘神州科技股份有限公司(Sino Biological Inc.)

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技术服务、试剂、抗体、细胞库 / 细胞培养、ELISA 试剂盒
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SEC-MALS验证的新冠突变株三聚体蛋白

人阅读发布时间：2021-09-29 14:31

SARS-CoV-2 Spike蛋白承担着病毒与宿主细胞膜受体结合及膜融合功能，是宿主中和抗体的重要作用位点以及疫苗设计的关键靶点。Spike蛋白含有2个不同功能的亚基：S1亚基通常呈球形，含有受体结合结构域，可与宿主受体结合；S2亚基促进病毒与宿主细胞的膜融合过程。

义翘神州已成功开发出高稳定性的三聚体S蛋白，涵盖了多种重要的新冠突变体，如Alpha（B.1.1.7）、Beta（B.1.351）、Delta（B.1.617.2）、Kappa（B.1.617.1）等。三聚体S蛋白的纯度、聚体形式和活性分别经过SDS-PAGE、SEC-MALS和ELISA进行验证。且经过若干次冻融和多批次测定对稳定性进行检测，结果表明已开发的三聚体S蛋白均具有高稳定。

SARS-CoV-2 Spike (D614G) Trimer (ECD, His tag)
(Cat: 40589-V08H8)

SEC-MALS Validated

The purity of SARS-CoV-2 Spike Trimer,His Tag (Cat. 40589-V08H8) was more than 90% and the molecular weight of this protein is around 400-540kDa verified by SEC-MALS.

ACE2 Binding (ELISA)		SDS-PAGE

Immobilized human ACE2 protein (mFc tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind spike trimer (Cat:40589-V08H8), the EC50 is 4-24 ng/mL.		Purity: > 90 %

SARS-CoV-2 B.1.617.2 Spike Trimer (ECD, His tag)
(Cat: 40589-V08H10)

SEC-MALS Validated

The purity of SARS-CoV-2 Spike Trimer, His Tag (Cat. 40589-V08H10) was more than 90% and the molecular weight of this protein is around 420-520kDa verified by SEC-MALS.

ACE2 Binding (ELISA)		SDS-PAGE

Immobilized human ACE2 protein (mFc tag) (Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind spike trimer (Cat:40589-V08H10), the EC50 is 4-24 ng/mL.		Purity: > 90 %

新冠突变体Spike三聚体蛋白列表

货号	WHO 命名	产品描述	突变位点
40589-V08H10	Delta	SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag)	T19R, E156G, 157Del, 158Del, L452R, T478K, D614G, D950N, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H11	Kappa	SARS-CoV-2 B.1.617.1 Spike S1+S2 trimer Protein (ECD, His tag)	G142D, E154K, L452R, E484Q, D614G, F817P, A892P, A899P, A942P, K986P, V987P, Q1070H, H1101D and furin cleavage site mutants
40589-V08H12	Alpha	SARS-CoV-2 B.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag)	(69-70, 145 deletion), N501Y, A570D, D614G, T716I, F817P, A892P, A899P, A942P, S982A, K986P, V987P, D1118H and furin cleavage site mutants
40589-V08H13	Beta	SARS-CoV-2 B.1.351 Spike S1+S2 trimer Protein (ECD, His tag)	L18F, D80A, D215G, 241-243 deletion, R246I, K417N, E484K, N501Y, D614G, A701V, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H8		SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag)	D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H9		SARS-CoV-2 B.1.618 Spike S1+S2 trimer Protein (ECD, His tag)	H49Y, 145-146 deletion, E484K, D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants